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Staining of Bacteria

Gram staining technique:

  1. First of all H. C. Gram differentiated bacteria on the basis of staining.

  2. In the first step of this method bacteria are stained with Crystal Violet and then KI solution.

  3. After staining, bacteria are washed with Acetone or Ethyl alcohol. After washing some bacteria retain the stain and some bacteria are decolorized.

  4. Bacteria which retain stain (violet or purple) are called Gram (+) and bacteria which… (36 more words) …

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