NEET MDS - Master of Dental Surgery (Medical) NEET-MDS Microbiology-Morphology of Bacteria Study Material (Page 3 of 3)
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ZN Staining
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Principle
- Bond of carbol fuchsine with bacterial lipid cell wall resist destaining with strong decolorizing agent such as alcohol and acids.
- Acid fast negative bacteria discharge stain when treated with alcohol solution.
- Counter staining is done with Methylene blue, the decolored acid-fast negative organism and other cells take blue colour in contrast with red-coloured acid fast organisms.
Reagents Used
Reagents used during this stain are Carbol-fuchsine solution, 20% sulphuric acid, and Methylene blue.
Procedure
- From sputum specimen prepare a smear on glass slide and heat fix it.
- On staining reck …
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