NEET MDS - Master of Dental Surgery (Medical) NEET-MDS Microbiology-Morphology of Bacteria Study Material (Page 3 of 3)

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ZN Staining

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Principle

  • Bond of carbol fuchsine with bacterial lipid cell wall resist destaining with strong decolorizing agent such as alcohol and acids.
  • Acid fast negative bacteria discharge stain when treated with alcohol solution.
  • Counter staining is done with Methylene blue, the decolored acid-fast negative organism and other cells take blue colour in contrast with red-coloured acid fast organisms.

Reagents Used

Reagents used during this stain are Carbol-fuchsine solution, 20% sulphuric acid, and Methylene blue.

Procedure

  • From sputum specimen prepare a smear on glass slide and heat fix it.
  • On staining reck …

… (93 more words, 10 figures) …

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