Morphogenesis-Protoplast Culture [IFS (Forests Services) Botany (Mains)]: Questions 1 - 6 of 6

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Question 1

Appeared in Year: 2015

Describe in Detail Essay▾

Briefly discuss the technique for raising haploids from microspores. (Paper 1, Section B)

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Explanation

Raising Haploids from Microspores

  • Haploid plants possess only a single set of chromosomes in the sporophyte.
  • Haploid plants are significance for the production of homozygous plants and for the improvement of plants in plant breeding programmers.

Production of Haploid Plants

Understanding the Production of Haploids

Haploids may be divided in two categori…

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Question 2

Appeared in Year: 2015

Describe in Detail Essay▾

Write short notes on the following: Protoplast fusion (Paper 1, Section B)

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Explanation

Protoplast Fusion

Protoplast fusion is a technique by which two protoplasts are fused to form hybrid cells that can grow into mature hybrid organisms.

Methods of Protoplast Fusion

Understanding the Method of Protoplast Fusion

Spontaneous Fusion

  • Protoplasts during isolation often fuse spontaneously and this phenomenon is called spontaneous fusion.
  • Simply …

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Question 3

Appeared in Year: 2013

Describe in Detail Essay▾

Write Short Notes on: Methods of protoplast fusion (Paper 1, Section B)

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Explanation

Methods of Protoplast Fusion

  • Protoplast fusion is the joining of two plant cells together to form one cell with multiple nuclei.
  • There are two methods of Protoplast fusion; Spontaneous fusion and induced fusion.
Image Showing Protoplast Fusion Methods

Spontaneous Fusion

  • Spontaneous fusion means protoplasts isolated fuse spontaneously.
  • Spontaneous fusion …

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Question 4

Appeared in Year: 2015

Describe in Detail Essay▾

Write short notes on the Protoplast fusion. (Paper 2)

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Explanation

Protoplast Fusion

  • Protoplast fusion is a type of genetic modification in the plants by which genome of two distinct species of plants are fused together to form new hybrid. That has characteristics of both the species.
  • Protoplast fusion can be obtain either by spontaneous fusion method or induced fusion methods.

Spontaneous Fusion Method

  • During isolati…

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Question 5

Appeared in Year: 2019

Describe in Detail Essay▾

How are protoplast isolated? Describe the methods of protoplast culture.

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Explanation

Protoplast

Protoplast is spherical naked plant cells produced by the removal of cell wall.

Protoplast Isolation

  • Removal of cell wall from plant cell by mechanical or enzymatic method is called protoplast isolation
  • Protoplast isolation can be done by two different methods

Mechanical Method

  • Large, highly vacuolated cells are used.
  • Cells are plasmolysis in a…

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Question 6

Appeared in Year: 2019

Describe in Detail Essay▾

Summarize the role of protoplast fusion in somatic hybridization.

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Explanation

  • Protoplast fusion is a type of genetic modification in the plants by which genome of two distinct species of plants are fused together to form new hybrid.
  • That has characteristics of both the species.
  • Protoplast fusion can be obtained either by spontaneous fusion method or induced fusion methods.

Spontaneous Fusion Method

  • During isolation protoplasts o…

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