Transgenic Animals (40 mins) | Lecture 43/90 | ASRB NET (ICAR-NET) Veterinary Microbiology
Video Course 2026 (90 Lectures- 67.5 hrs): Offline Support
Welcome back! Your subscription is active. Please access it from list of lectures.%
Study Material (212 Notes): 2026-2027 Syllabus
Welcome back! Your subscription is active. Please access it from list of detailed notes%
519 PYQs with Explanations (2026-2027 Exam)
Welcome back! Your subscription is active. Please access it from questions with explanations%
Bookmarked Times
Details
Techniques in Microbiology and VaccinologyApplication of Molecular Techniques
Transgenic Animals
Transgenesis: Adding foreign DNA heritably.
Transgenic Animals: Engineered with foreign DNA.
Genetic Modification: Altering genetics.
Genetic Engineering: Modifying DNA.
Transgenic Organism: Engineered with foreign genes.
Production of transgenic animals
Microinjection: DNA into male pronucleus.
Engineered stem cells into early embryos.
Retroviral vectors in early embryo cells.
Gene knockout
Gene Knockout: Organism with non-working genes.
Difference from Transgenic Mice: Transgenic mice have extra genes; knockouts have non-functional native genes.
Difference from Knock-In Mice: Knockouts had genes replaced; knock-ins add new genes.
Knock-In Mice: Insert functional genes, sometimes from other species.
Cre-loxP recombinant system
Nuclear Transfer Technology
Principle: Oocyte nucleus replaced with somatic cell nucleus.
Development:
First in Xenopus (1952), Dolly (1995).
Procedure:
Enucleation, nucleus transfer, embryo activation.
Applications:
Cloning, genetic modification, livestock improvement.
Question: ANDi is a cloned?
Answer: genetically modified Rhesus monkey
EditYour device connection seems slow, switching to low quality for smoother playback. You might want to cache the lecture for better experience.